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I use with success a diverse range of a cutting edge technology evidenced for my publications (PLoSOne,GenesBrainBehavior, Neurogenetics, JournalBiologicalChemistry, AgingCell, ScientificReports). Molecular approaches: genome editing by CRISPR/Cas9, molecular cloning. Transgenic methods: creation of transgenic lines by microinjection for gene expression, rescue lines. Microscopy technologies: live imaging using Differential Interface Contrast, fluorescence or confocal optical, precise microscopy approaches for celular identification (neurons and non-neuronal cells), sub-cellular distribution. Cellular approaches: neuroanatomy in the C. elegans simple nervous system, dynamic Ca2+ imaging using genetically encoded reporters in freely moving animals. Electrophysiology: extra and intracellular recordings, electropharyngeograms resolving electrophysiological features. Nano-engineering technologies: Microfluidic devices (lab-on-a-chip) for electrophysiology and behaviours
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